Gel-free and Gel-based Proteomics in Bacillus subtilis
نویسندگان
چکیده
منابع مشابه
Gel-free and gel-based proteomics in Bacillus subtilis: a comparative study.
The proteome of exponentially growing Bacillus subtilis cells was dissected by the implementation of shotgun proteomics and a semigel-based approach for a particular exploration of membrane proteins. The current number of 745 protein identifications that was gained by the use of two-dimensional gel electrophoresis could be increased by 473 additional proteins. Therefore, almost 50% of the 2500 ...
متن کاملgel-based proteomics
proteomics has emerged since 1995 for studying the total proteome of cells, tissues, or organisms at any condition. the study of proteins has a fundamental importance because they are the functional and final molecules of the gene expression process and proteomics could be a valuable technique for achieving this purpose. mass spectrometry is a complementary technique for proteomics that allows ...
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Two-dimensional gel electrophoresis (2-DE) is widely applied and remains the method of choice in proteomics; however, pervasive 2-DE-related concerns undermine its prospects as a dominant separation technique in proteome research. Consequently, the state-of-the-art shotgun techniques are slowly taking over and utilising the rapid expansion and advancement of mass spectrometry (MS) to provide a ...
متن کاملGel-based proteomics: what does MCP expect?
Almost three decades have elapsed since O’Farrell and Klose independently introduced high-resolution two-dimensional polyacrylamide gel electrophoresis (2D-PAGE)—often referred to as gel-based proteomics—as a core technology for analyzing the protein composition of cells, tissue, or bio fluids. Since then, many technological developments have taken place leading to improvements in the procedure...
متن کاملGel-free proteomic identification of the Bacillus subtilis insoluble spore coat protein fraction.
Species from the genus Bacillus have the ability to form endospores, dormant cellular forms that are able to survive heat and acid preservation techniques commonly used in the food industry. Resistance characteristics of spores towards various environmental stresses are in part attributed to their coat proteins. Previously, 70 proteins have been assigned to the spore coat of Bacillus subtilis u...
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2006
ISSN: 1535-9476
DOI: 10.1074/mcp.m600069-mcp200